Disseminated cryptococcosis initially presenting as cellulitis in a patient suffering from nephrotic syndrome
© Ni et al.; licensee BioMed Central Ltd. 2013
Received: 3 August 2012
Accepted: 18 January 2013
Published: 22 January 2013
Cryptococcosis is considered as an opportunistic infection because it mainly occurs in immunosuppressed hosts. C. neoformans is usually acquired by the respiratory route and then may disseminate hematogenously to other places, such as meninges, bone and skin. Cutaneous involvement is a rare but important feature of disseminated cryptococcosis with a poor outcome if misdiagnosis. We reported the first case of patients with nephrotic syndrome suffering from disseminated cryptococcosis initially presented as cellulitis.
A 34-year-old man developed severe cellulitis on his both lower extremities without any preceding injury and allergies. The patient had been treated with systemic corticosteroids nearly one year for nephrotic syndrome. According to the outcome of blood culture, the wound area was interpreted as bacterial cellulitis at first. However, the antimicrobial treatment made no response and the skin biopsy revealed the presence of Cryptococcus neoformans, which was subsequently confirmed by microbiological culture. Though the initiation of therapy with fluconazole 400 mg per day was immediately adopted, the patient’s conditions suddenly plummeted and he died in the end.
Since the poor outcome of disseminated cryptococcosis if unrecognized and untreated in time, it should be investigated rigorously as a differential diagnosis in patients with nephrotic syndrome suffering from cutaneous diseases.
KeywordsCutaneous cryptococcosis Cellulitis Nephrotic syndrome Immunosuppression
Cryptococcus neoformans, a non-mycelial encapsulated budding yeast characterized by producing a teleomorph state (the sexual stage) and an anamorph state (asexual reproductive stage), are important opportunistic pathogens that usually infect immunosuppressed individuals . The most common exposure sources are inhalation of aerosolized infectious yeast from pigeon, avian excreta, contaminated soil, milk, fruits and wood products [2, 3]. These organisms then may hematogenously disseminate to other places, mainly the central nervous system . cryptococcal skin disease is a rare feature of disseminated cryptococcosis, and has a poor outcome if unrecognized and untreated . Here, we present a case of cryptococcal cellulitis in a patient with nephrotic syndrome who was receiving long-term steroid treatment. Reviewing the literature, this is the first report of nephrotic syndrome with disseminated cryptococcosis initially presented as cellulitis.
On examination, the both inner thigh exhibited massive necrotic superficial ulceration overlying erythema, each approximately 20 cm in diameter; the forearms exhibited several reddish indurations. No regional lymphnodes were involved. Urinalysis revealed proteinuria (5 g/L) and haematuria (15/HPF). The 24 h urine protein was 4.15 g and the plasma albumin was 24.7 g/L. The total serum cholesterol and serum triglycerides were 7.56 mmol/L and 3.02 mmol/L, respectively. Blood urea nitrogen and creatinine were 100 mg/dl and 12.5 mg/dl, respectively. In addition, the anti-HIV antibodies, anti-citrullinated peptide antibodies (ACPA), anti-nuclear antibodies (ANA), and anti-neutrophil cytoplasmic antibodies (ANCA) were all tested negative. The initial chest X-ray revealed a normal finding.
On the day of admission, we initiated an empirical treatment with moxifloxacin and cefmenoxime intravenously due to the assumed bacterial cellulitis. In addition, Gamma globulin was added for enhancing his immunity and 60 mg of prednisolone daily was still administrated for the nephrotic syndrome. Blood cultures were obtained in order to clarify the pathogen. Considering the potential inflammation dissemination caused by skin biopsy, we did not do it initially.
Three days later, the cutaneous lesions had slightly reduced with the high fever continuous existence. The outcome of blood culture was coagulase negative staphylococci and the X-ray of the lung revealed a change of ground-glass opacity in both perihilar regions. Treatment regimen changed to Linezolid plus Caspofungin through the outcome of blood culture. However, the patient has little response to the treatment with the appearance of gradually blurred consciousness, delirium, and hypoxemia. Then he was transferred to respiratory intensive care unit for further therapy.
Unfortunately, on the 6th day, the patient suddenly appeared the signs of acute respiratory failure with intractable hyoxemia and the mechanical ventilation was initiated. But then acute heart failure occurred. Despite timely rescue, his blood pressure was still at a quite low level maintained by dopamine accompanied with oliguria. The patient died on the eight day of admission.
Immunologic impairment can frequently occur in patients with nephrotic syndrome because of low serum immunoglobulin G concentrations, reduced complement activity, depressed T-cell function and the use of corticosteroids or cytotoxic agents . The immunologic impairment, adding the protein-rich edematous skin serving as a good media, increases the vulnerability to various cutaneous infections  (be reported in up to 27.02% ), particularly the cellulitis. The most common pathogens that cause cellulitis are β-hemolytic Streptococci and some gram-negative bacteria such as E.coli . But other atypical organisms shouldn’t be neglected considering the immunocompromised condition in nephrotic syndrome patients just as our case.
As important opportunistic pathogens, C. neoformans usually infect individuals with AIDS. Nevertheless, with an increasing number of steroid-dependent individuals as our patient in the last decade, the prevalence rate of cryptococcosis is continuously rising . In humans, C. neoformans usually cause three types of infections: pulmonary cryptococcosis, cryptococcal meningitis and wound or cutaneous cryptococcosis . Cutaneous involvement, a rare but important feature of disseminated cryptococcosis, may precede systemic symptoms by as long as 8 months . Naoko Ogami has report a patient with nephrotic syndrome suffering from primary cutaneous cryptococcosis without other organ involvement . However, cutaneous cryptococcal disease usually represents the hematogenous dissemination of cryptococcosis. Its presentation has various clinical morphologies: a draining sinus tract, verrucous nodules, molluscum-like lesions and erythematous, indurated plaques, and so on . Yet, none of these manifestations are pathognomonic for cryptococcal infection. Confusion with other resemble diseases is not uncommon and a diagnostic delay might be a major factor contributing to high associated mortality . A timely histological analysis of skin biopsies can assist to confirm the pathogens. In addition, the importance of CSF cultures and serological studies should not be over emphasized .
According to the current guidelines of the Infectious Diseases Society of America (IDSA), amphotericin B combined with flucytocine is recommended as primary therapy regimen for disseminated cryptococcosis followed by fluconazole as consolidation therapy . To those with renal dysfunction, lipid formulations of AmB or AmB lipid complex could replace Am B to avoid further deterioration of the renal function. With appropriate systemic antifungal therapy, 74% of patients with cryptococcal cellulitis can be expected to survive in immunocompetent status . However, to those having developed into organ failures, as seen in our patients, a review of the literature suggests a much graver prognosis. The mortality rate of ARF result from disseminated cryptococcosis was 100% and 55% respectively in immunocompromised patients with or without AIDS [5, 18], and nearly all deaths occurred within 2 weeks of diagnosis with or without treatment indicating an aggressive and fulminant progress of the disease.
There are several potential explanations for the poor prognosis of our patients. First, a severe disseminated cryptococcal infection that caused multiple organ failure lead to the poor outcome. Second, the delayed diagnosis and antifungal therapy have also been a contributing factor. Finally, the patients’ suddenly deteriorated condition after two day’s antifungal therapy reminded that immune reconstitution inflammatory syndrome (IRIS) might also have contributed to his death. The IRIS which manifests with inflammatory reactions, targeted at the antigens of opportunistic infections can occur in immunocompromised patients after some degree of immune restoration . If the IRIS occurs in patients already diagnosed with opportunistic infections, it may result in recurrence or worsening of clinical features despite effective treatment . It has been reported that in patients with cryptococcal meningitis, antifungal therapy may prompt the reversion of an immunosuppressive Th2 response to the normally protective Th1 cytokine response, leading to an exuberant host response against residual sites of disease . Because we didn’t monitor the changes of T cells in our patient, the IRIS as a risk factor can’t be excluded completely.
In conclusion, this case demonstrated that C. neoformans should be considered in the differential diagnosis of cutaneous infection in nephrotic patients or other immunosuppressive patients. Even when the organism is clear, one can't be too cautious to reconsider the potential existence of other atypical pathogens if the initial treatment makes little effects.
We thank the following for critical comments on the manuscript: Parisa Badiee, Emanuele Cozzani, Inês Ferreira.
- Magee JT, Philopt C, Yang J, Hosein IK: Pyrolysis typing of isolates from a recurrence of systemic cryptococcosis. J Med Microbiol. 1994, 40: 165-169. 10.1099/00222615-40-3-165.View ArticlePubMedGoogle Scholar
- Lazera MS, Cavalcanti MA, Trilles L, Nishikawa MM, Wanke B: Cryptococcus neoformans var.gattii–evidence for a natural habitat related to decaying wood in a pottery tree hollow. Med Mycol. 1998, 36: 119-122.PubMedGoogle Scholar
- Haag-Wackernagel D, Moch H: Health hazards posed by feral pigeons. J Infect. 2004, 48: 307-313. 10.1016/j.jinf.2003.11.001.View ArticlePubMedGoogle Scholar
- Huston SM, Mody CH: Cryptococcosis: an emerging respiratory mycosis. Clin Chest Med. 2009, 30: 253-264. 10.1016/j.ccm.2009.02.006.View ArticlePubMedGoogle Scholar
- Visnegarwala F, Graviss EA, Lacke CE, et al: Acute respiratory failure associated with cryptococcosis in patients with AIDS. Clin Infect Dis. 1998, 27: 1231-1237. 10.1086/514984.View ArticlePubMedGoogle Scholar
- Se Jin P, Jae Il S: Complications of nephrotic syndrome. Korean J Pediatr. 2011, 54: 322-328. 10.3345/kjp.2011.54.8.322.View ArticleGoogle Scholar
- Lee KW, Kim SS, Jeong MS, Lee JH, Park JK: Shin YT: spontaneous cellulitis in nephrotic syndrome. Clin Nephrol. 1995, 43: 133-134.PubMedGoogle Scholar
- Moorani KN, Khan KM, Ramzan A: Infections in children with nephrotic syndrome. J Coll Physicians Surg Pak. 2003, 13: 337-339.PubMedGoogle Scholar
- Gunderson CG: Cellulitis: Definition, Etiology, and Clinical Features. Am J Med. 2011, 124: 1113-1122. 10.1016/j.amjmed.2011.06.028.View ArticlePubMedGoogle Scholar
- Harris JR, Lockhart SR, Debess E, Marsden-Haug N, Goldoft M, Wohrle R, Lee S, Smelser C, Park B, Chiller T: Cryptococcus gattii in the United States: Clinical Aspects of Infection With an EmergingPathogen. Clin Infect Dis. 2011, 53: 1188-1195. 10.1093/cid/cir723.View ArticlePubMedGoogle Scholar
- Probst C, Pongratz G, Capellino S, Szeimies RM, Schölmerich J, Fleck M, Salzberger B, Ehrenstein B: Cryptococcosis mimicking cutaneous cellulitis in a patient suffering from rheumatoid arthritis: a case report. BMC Infect Dis. 2010, 10: 239-10.1186/1471-2334-10-239.View ArticlePubMedPubMed CentralGoogle Scholar
- Rico MJ, Penneys NS: Cutaneous cryptococcosis resembling molluscum contagiosum in a patient with AIDS. Arch Dermatol. 1985, 121: 901-902. 10.1001/archderm.1985.01660070091023.View ArticlePubMedGoogle Scholar
- Ogami N, Horino T, Arii K, Takao T, Hashimoto K, Seike M, Kodama H: Primary cutaneous cryptococcosis in a patient with minimal change nephrotic syndrome. Nephrol Dial Transplant. 2005, 20: 2578-2579. 10.1093/ndt/gfi047.View ArticlePubMedGoogle Scholar
- Dimino-Emme L, Gurevitch AW: Torrance: Cutaneous manifestations of disseminated cryptococcosis. J Am Acad Dermatol. 1980, 32: 844-850.View ArticleGoogle Scholar
- Taneja J, Bhargava A, Loomba P, Dogra V, Thakur A, Mishra B: Cryptococcal granulomas in an immunocompromised HIV-negative patient. Indian J Pathol Microbiol. 2008, 51: 553-555. 10.4103/0377-4929.43760.View ArticlePubMedGoogle Scholar
- Perfect JR, Dismukes WE, Dromer F, Goldman DL, Graybill JR, Hamill RJ, Harrison TS, Larsen RA, Lortholary O, Nguyen MH, Pappas PG, Powderly WG, Singh N, Sobel JD, Sorrell TC: Clinical practice guidelines for the management of cryptococcal disease: 2010 update by the infectious diseases society of America. Clin Infect Dis. 2010, 50: 291-322. 10.1086/649858.View ArticlePubMedGoogle Scholar
- Pappas PG, Perfect JR, Cloud GA, Larsen RA, Pankey GA, Lancaster DJ, Henderson H, Kauffman CA, Haas DW, Saccente M, Hamill RJ, Holloway MS, Warren RM, Dismukes WE: Cryptococcosis in Human Immunodeficiency Virus–Negative Patients in the Era of Effective Azole Therapy. Clin Infect Dis. 2001, 33: 690-699. 10.1086/322597.View ArticlePubMedGoogle Scholar
- Vilchez RA, Linden P, Lacomis J, Costello P, Fung J, Kusne S: Acute Respiratory Failure Associated With Pulmonary Cryptococcosis in Non-AIDS Patients. Chest. 2001, 119: 1865-1869. 10.1378/chest.119.6.1865.View ArticlePubMedGoogle Scholar
- Elston JW, Thaker H: Immune reconstitution inflammatory syndrome. Int J STD AIDS. 2009, 20: 221-224. 10.1258/ijsa.2008.008449.View ArticlePubMedGoogle Scholar
- Meintjes G, Scriven J, Marais S: Management of the immune reconstitution inflammatory syndrome. Curr HIV/AIDS. 2012, 9: 238-250. 10.1007/s11904-012-0129-5.View ArticleGoogle Scholar
- Ecevit IZ, Clancy CJ, Schmalfuss IM, Nguyen MH: The poor prognosis of central nervous system cryptococcosis among nonimmunosuppressed patients: a call for better disease recognition and evaluation of adjuncts to antifungal therapy. Clin Infect Dis. 2006, 42: 1443-1447. 10.1086/503570.View ArticlePubMedGoogle Scholar
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