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Table 3 Ratio of mRNA abundance in glomeruli compared to lung, spleen and liver.

From: A human glomerular SAGE transcriptome database

    SAGE RT-PCR
Symbol Name LongTag G: TPM P: TPM Ratio G: P G: Lu G: Sp G: Li
SOST Sclerosteosis ACATATGAAAGCCTGCA 82 0.00 Infinity 130 ± 48 13007 ± 4827 7969 ± 2957
TCF21 Transcription factor 21 CGAGTGCTGAGCAAGGC 817 1.59 514 49 ± 16 27 ± 9 384 ± 123
FGF1 Fibroblast growth factor 1 (acidic) TAAAGGCCTTTAATAAG 102 0.49 210 490 ± 70 166 ± 23 2565 ± 367
SPOCK2 sparc/osteonectin, cwcv and kazal-like domains TGTGGAGTGTACTTGTT 245 1.44 170 45 ± 13 39 ± 12 258 ± 75
PTHR1 Parathyroid hormone receptor 1 TGACCAGGCGCTGGGGG 1143 8 141 196 ± 55 51 ± 14 30 ± 8
EFNB1 Ephrin-B1 AGGGAAGAGGAAAGTGC 102 1.50 68 20 ± 4 17 ± 3 30 ± 6
CDKN1C Cyclin-dependent kinase inhibitor 1C (p57, Kip2) TAGCAGCAACCGGCGGC 1021 46 22 51 ± 17 45 ± 15 198 ± 66
IGFBP5 Insulin-like growth factor binding protein 5 GAGTACGTTGACGGGGA 367 18 20 10 ± 4 22 ± 9 43 ± 17
CLDN5 Claudin 5 GACCGCGGCTTCCGCCG 715 38 19 5 ± 1 27 ± 7 24 ± 6
FAM65A Family with sequence similarity 65, member A GGTTCCTGGTGCCCCTT 755 43 17 18 ± 5 11 ± 3 51 ± 15
FOXC1 Forkhead box C1 AGCCTGTACGCGGCCGG 245 15 17 25 ± 6 302 ± 74 452 ± 110
C9orf58 Chromosome 9 open reading frame 58 GGAGTGTGCGTGGACTG 1572 102 15 36 ± 3 2 ± 0 171 ± 16
NES Nestin TGCTGACTCCCCCCATC 2001 138 14 22 ± 6 23 ± 6 339 ± 84
VEGFA Vascular endothelial growth factor A TTTCCAATCTCTCTCTC 1450 109 13 16 ± 4 53 ± 12 22 ± 5
ZDHHC6 Zinc finger, DHHC-type containing 6 TGGTACTTCTCTTTTCC 592 51 12 Infinity 60 ± 18 Infinity
MYO1E Myosin IE TATGAATGTACTAAGTA 245 26 9 11 ± 3 21 ± 6 13 ± 3
MYL9 Myosin, light chain 9, regulatory GGAGTGTGCTCAGGAGT 3287 454 7 4 ± 1 15 ± 4 45 ± 11
ITM2B Integral membrane protein 2B TCACCTTAGGTAGTAGG 3328 508 7 4 ± 1 4 ± 1 5 ± 1
MYO1D Myosin ID ATTGTAGACAATGAGGG 327 82 4 3 ± 0 7 ± 1 7 ± 1
  1. The SAGE tag frequency in glomeruli (G: TPM) and SAGE library pool (P: TPM) as well as the relative SAGE tag enrichment in glomeruli (Ratio G: P) is shown. The transcript abundance relative to lung (G: Lu), spleen (G: Sp) and liver (G: Li) was determined by RT-PCR using distinct sets of microdissected glomeruli from kidneys of three different donors. G: glomeruli, Lu: lung, Sp: spleen, Li: liver. Mean ± SEM.