Figure 1

Genotyping of C1564S, L1649R, and R1677Q by melting curve analysis. Each graph analyzes one of the mutations with DNA from a positive male (dotted line), a female carrier (black line), a normal individual (grey line) and a no template control (thin grey line). Fluorescence on the Y axis is the negative derivative of fluorescence in function of temperature. Positions of the peaks indicate the dissociation of the probes with a given allele. As probes are homologous to the normal allele, lower T _ms correspond to the mutant alleles and higher T _ms to the normal allele.