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Figure 3 | BMC Nephrology

Figure 3

From: Over-expression of adenosine deaminase in mouse podocytes does not reverse puromycin aminonucleoside resistance

Figure 3

Characterization of ADA transgenic mice. A. Western blot analysis of ADA expression. Expression of ADA was analyzed using an anti-HA antibody in glomerulus (Gl), proximal convoluted tubule (PCT) and cortical collecting duct (CCD) from two mice from lines 2 (2.1 and 2.3) and 3 (3.6 and 3.9). Glomerulus from two wild type mice (WT) served as negative controls. Only mice from line 2 expressed ADA, and expression was restricted to the glomerulus. B. ADA activity in mouse and rat kidney. ADA activity was determined in kidney homogenates from wild type mice, ADAtg mice and control rats. Values (units/mg tissue protein/min) are means ± SE from several samples (number indicated above columns). Statistical differences between groups were assessed by variance analysis: ***, p < 0.001.

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