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Figure 11 | BMC Nephrology

Figure 11

From: Bone Morphogenetic Protein (BMP)-7 expression is decreased in human hypertensive nephrosclerosis

Figure 11

BMP-7 deceases TNF-α induced apoptosis. Induction of apoptosis (A) and cell death (B) in proximal tubular cells (HK-2) after stimulation with 20 ng/ml TNF-α alone and/or in combination with BMP-7 (1, 10, 100 ng/ml). Apoptotic cells were detected by FACS analysis counting annexin-V positive and propidium iodide negative cells (n = 3). Cell death was detected after staining with annexin-V (n = 3). TNF-α alone induced high rates of apoptosis and cell death. Co-stimulation with TNF-α and BMP-7 resulted in significant dose-dependent lower rates of apoptosis and cell death compared to TNF-α alone (DIF: p < 0.05, FACS: p < 0.05). The results were analysed by Student's t-test and are displayed as the % of unstimulated cells.

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