Figure 6
From: Bone Morphogenetic Protein (BMP)-7 expression is decreased in human hypertensive nephrosclerosis
BMP-7 mRNA level in HK-cells is decreased after stimulation with angiotensin-II and increased after stimulation with telmisartan. BMP-7 mRNA level in the proximal tubule cell line HK-2 was determined by real-time PCR (housekeeping gene PPIA) after stimulation with angiotensin-II (10-7, 10-5, 10-3 M) and telmisartan (10, 20, 30 μM) or with both combined for 12 hours (n = 12). BMP-7 mRNA level is significantly decreased after stimulation with 10-3 M AT-II (p < 0.001) and increased after stimulation with 30 μM telmisartan (p < 0.01). Co-stimulation of AT-II and telmisartan showed non-significantly increased BMP-7 mRNA levels compared to stimulation with AT-II alone. The results are presented as the mean % of unstimulated cells ± standard error.