Figure 5

Analysis of neovascularization. (A) Agiogenesis of BM-derived CD105⁺ endothelial cells and confirmed by GFP co-expression (arrowhead). Scale bars = 50 μm (upward) and 20 μm (below), respectively. (B) Immunostaining of α-SMA and GFP demonstrating functional vessels formation of BM derived cells (arrowhead). Nuclear was stained with DAPI. Scale bars = 50 μm (upward) and 5 μm (below), respectively. (C & D) Quantitative analysis revealed G-CSF administration can increase capillary density significantly comparing to sham and saline groups. Nuclear was stained with DAPI. Scale bar = 50 μm.