Figure 3

MT1-MMP induces cell proliferation and cyst growth in 3D collagen I gel culture. A and B: RT-PCR (A) and western blot (B) analyses of the MT1-MMP mRNA and protein expression in parental and MT1-MMP overexpressed MDCK cells. C and D: Western blot analysis of the PCNA expression in parental and MT1-MMP overexpressed MDCK cells that were cultured under 2D (C) or 3D (D) conditions. E. The diameters of the cysts that were formed from parental MDCK cells or MT1-MMP overexpressing MDCK cells treated with or without Doxycycline for 9 days in 2.5 mg/ml collagen I gels. **p < 0.005 versus MDCK cells. *p < 0.005 versus MDCK MT1-MMP cells.