Expression of NGAL mRNA in the LPS-treated rats. A, In situ hybridization signal time-course. A strong hybridization signal was observed in the cortical tubular epithelia in samples in the 3-, 6- and 12-hour LPS groups compared with a weak signal in the 1- and 24-hour LPS groups, and there was no signal in the controls. B, Relative expression of NGAL mRNA (relative to the controls) is shown. C, Agarose gel electrophoresis imprinting expresses the intensity of NGAL mRNA in the LPS-treated rats. There was a 129-fold increase in NGAL mRNA expression at 3 hours after LPS treatment, rising to 226-fold at 6 hours and then it decreased to 162-fold at 12 hours. **P < 0.001, relative to the control group.