Glomerular changes in db/db diabetic mice. Panels A, C and E are controls, and panels B, D and F are from db/db mice with diabetic nephropathy. Panels A and B show histological sections stained with hematoxylin and eosin and demonstrate glomeruli hypertrophy. Panels C and D are sections stained with Masson's trichrome stain. Note the increased staining (blue) indicating a higher level of collagen deposition in the glomeruli of diabetic mice. Panels E and F are sections stained with Periodic acid-Schiff (PAS) and show mesangial expansion in diabetic mice. All images are representative glomeruli at an original magnification of 200X.