Figure 1

PCR for the Sry gene confirms the engraftment of mutant bone marrow-derived cells in wild type mice. PCR-based detection of the male-specific Sry gene in peripheral blood confirmed the engraftment of male bone marrow cells in female mice 4 weeks after transplantation. An 800 base pair band is present in all genetically normal females transplanted with male mutant bone marrow (Lanes1-6) and a male positive control (Lane 7), but is absent from an untransplanted female negative control (Lane 8).