Figure 3

Mutant Y chromosome positive cells derived from bone marrow are present in recipient kidneys. (A and B) Representative fluorescence micrographs of Y chromosome-positive cells in the kidney are shown for a wild type male control (A) and a female recipient of male mutant PKD BM 4 weeks post IR (B). Nuclei are counterstained with DAPI (blue). (C) Quantification of Y chromosome-positive cells per field in transplanted females showed that BM-derived cells with the PKD mutation were detectable 2, 4 and 12 weeks post IR. Percentages of Y chromosome positive cells are shown as mean ± SEM for at least 10 fields and a total of 500 nuclei per time point. Sections assessed were from 2 mice at 2 weeks, 3 mice at 4 weeks and 2 mice at 12 weeks.