Figure 1

Differentially expressed proteins from renal cortex of sham operated and PNx rats. Representative images of 1 from 10 two-dimensional gels run for every protein extract from the control and PNx groups. 2-D gels were stained with Silver. A – in cytosolyc fraction (Extract 1) the most prominent spots with decreased levels in cortex of PNx rats were identified as MMSDH in spot 1, and PGK1, MCAD and aginonosuccunate synthase 1 in the spot 2. Protein in spot 3 was identified as 40 kD fragment of fibrosis marker protein vimentin. B – in hydrophobic fraction (Extract 2) the most prominent difference between proteins from PNx and sham operated rats were seen in spots 4 and 5. These proteins were identified as albumin (a marker of tubulointerstitial injury) and GRP-75 (a mitochondrial chaperone molecule) respectively. C – in the fraction of membrane-bound proteins (Extract 3) of renal cortex, the protein from spot 6 was identified as gamma subunit of ATP-1 synthase.