Figure 4

Mitochondrial biogenensis and autophagy in the cortical tissue of PNx rats. Representative immunoblot (A) and densitometry analysis (B) of expression of proteins controlling mitochondrial biogenesis – PGC1α and PPARγ (A,B). No significant changes were found. n = 10. Representative western blots (C) and densitometry analysis (D) of Beclin 1 and BNIP3 demonstrate significant up-regulation of these proteins in PNx kidneys when compared with sham-operated controls. Note two forms of LC3: slowly moving cytoplasmic LC3-I, and more mobile lipidated form of LC3-II (A). n = 8, *p < 0.05.