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Table 2 Renal proteins identified by MALD-TOF mass spectrometry and some parameters used to evaluate SEQUEST search results

From: Mitochondrial impairment in the five-sixth nephrectomy model of chronic renal failure: proteomic approach

Spot number

Protein name

Accession number

Molecular weight

Protein coverage by amino /acid mass (%)

Peptide count

XCorr value ± SD

Delta correlation value ± SD

Rank /Sp ± SD

Ions (%) ± SD

Localization/function

1

Methylmalonate Semialdehyde dehydrogenase

giI13591997

57770

56.0/56.2

13

3.63 ± 0.93

0.45 ± 0.11

1.23 ± 1.09

67.26 ± 16.52

Mitochondria/ TCA, valine and pyrimidine metabolism

2

Phosphoglycerate kinase 1

giI1730519

44534

44.6/45.2

15

3.36 ± 1.12

0.32 ± 0.16

1.66 ± 2.09

59.31 ± 17.60

Cytoplasm/glycolysis

2

Acyl-coenzyme-A-dehydrogenase (medium chain)

giI8392833

46535

39.0/39.0

13

3.44 ± 1.03

0.37 ± 0.14

1.07 ± 0.27

66.61 ± 9.3

Mitochondria/ β-oxidation

2

Arginosuccinate synthetase 1

giI25453414

46467

55.1/55.7

24

3.80 ± 1.22

0.37 ± 0.15

1 ± 0

65.80 ± 21.33

Cytoplasm/urea cycle

3

Vimentin (fragment)

giI14389299

53697

50.1/50.0

23

3.53 ± 1.18

0.34 ± 0.11

1.26 ± 1.05

76.11 ± 18.50

Cytoplasm/cytoskeleton

4

Albumin (rat)

giI19705431

68674

73.8/73.0

45

3.84 ± 0.79

0.40 ± 0.11

1.06 ± 0.33

71.63 ± 14.14

Plasma/ protein transport

5

Glucose regulated protein 75

giI2119726

73699

53.2/53.1

31

3.97 ± 0.71

0.44 ± 0.11

1 ± 0

72.68 ± 11.90

Mitochondria/chaperon, structural

6

Gamma subunit ATP-1 synthase

giI6729936

29933

36.7/35.9

8

3.49 ± 0.72

0.47 ± 0.10

1.12 ± 0.35

49.42 ± 24.24

Mitochondria/oxidative phosphorylation