Figure 2

Effect of metabolic stress on cytosolic ATP levels in MPT cells from α1 ^-/- and α2 ^-/- KO mice. MPT cells from α1^-/- mice or α2^-/- KO mice, or their WT controls, were subjected to graded ATP depletion by incubation in the presence of antimycin (2 μM) and varying concentrations of dextrose for 4 hrs. Cell ATP levels were measured and normalized to cell protein content. All values are expressed as a percent of ATP levels in MPT cells incubated under control conditions (10 mM dextrose, in the absence of antimycin). * p < 0.02, antimycin plus 10 mM dextrose vs. control conditions; # p < 0.01, antimycin plus 5 mM dextrose vs. antimycin plus 10 mM dextrose; p < 0.01, antimycin plus 2.5 mM dextrose vs. antimycin plus 5 mM dextrose.