Effect of metabolic stress on activation of the AMPK pathway in MPT cells from α1
KO mice. MPT cells obtained from α1-/- or α2-/- KO mice, or their WT controls, were subjected to metabolic stress by incubation in medium containing dextrose (5 mM) plus antimycin (2 μM) for 4 hrs. Control cells were incubated in dextrose in the absence of antimycin. Activation of the AMPK pathway was assessed by immunoblotting using antibodies that recognize either the phosphorylated (activated) form of AMPK or the phosphorylated (inhibited) form of acetyl coenzyme A carboxylase (ACC). Expression of total AMPK was also assessed. Upper panel: Representative immunoblot. Molecular weight markers (in kDa) are shown on the right of each immunoblot. Lower panel: Densitometric quantitation of three immunoblots using β-actin as a loading control. * p < 0.001, presence vs. absence of antimycin in MPT cells from α1-/- mice, α2-/-KO mice and their WT controls.