Effect of pharmacologic inhibition of AMPK using compound C (CC) on the activity of the AMPK pathway in MPT cells from α2
KO and WT mice. MPT cells from α2-/- mice, or their WT control, were subjected to ATP depletion by incubation in medium containing dextrose (5 mM) and antimycin (2 μM), in the presence or absence of CC (20 μM) for 4 hrs. Activation of the AMPK pathway was assessed by immunoblotting, using antibodies that recognize either the phosphorylated (activated) form of AMPK, or the phosphorylated (inhibited) form of ACC. Expression of total alpha domain was also assessed. Upper panel: Representative immunoblot. Molecular weight markers (in kDa) are shown on the right of each immunoblot. Lower panel: Densitometric quantitation of three immunoblots using β-actin as a loading control. * p < 0.01, presence vs. absence of CC, for MPT cells from WT mice treated with antimycin; # p < 0.01, presence vs. absence of CC, for MPT cells from α2-/- mice treated with antimycin.