Effect of pharmacologic inhibition of AMPK on viability of MPT cells from α2
KO and WT mice subjected to metabolic stress. A: MPT cells from α2-/- mice, or their WT control, were subjected to graded ATP depletion by incubation in the presence of antimycin (2 μM) and varying concentrations of dextrose for 16–18 hrs, in either the absence (solid lines) or the presence (dashed lines) of CC (20 μM). The percentage of viable cells remaining was determined by flow cytometry (n = 5). * p < 0.01, absence vs. presence of CC, for MPT cells from either α2-/- mice or their WT control (ANOVA for repeated measures). B: Representative flow cytometric analysis of metabolically stressed MPT cells from α2-/- mice incubated in the absence (left panels) or presence (right panels) of CC (20 μM). Cell viability was quantified by staining cells with calcein AM (x-axis) and ethidium homodimer-1 (Eth-H1) (y-axis). For each condition, 10,000 cells were analyzed, and the percentage of viable cells was calculated by dividing the number of calcein-positive and Eth-H1-negative cells by the total number of cells counted.