Effect of knockdown of the α2 isoform on expression of the α1 and α2 isoforms and of total alpha domain in MPT cells from α1
and WT mice. MPT cells from α1-/- mice, or their WT control, were infected with control (scrambled) shRNA or anti-α2 shRNA designed to knock down the expression of the α2 isoform of AMPK. The relative expression of the α1 and α2 isoforms of AMPK, as well as of the total α domain (α1 + α2), was evaluated in immunoblots of lysates from confluent MPT cells derived from the kidneys of α1-/- and α2-/- mice, and their WT controls. Upper panel: Representative immunoblot. Molecular weight markers (in kDa) are shown om the right of each immunoblot. Lower panel: Densitometric quantitation of three immunoblots using β-actin as a loading control. * p < 0.05, anti-α2 shRNA vs. control shRNA, for α2 isoform expression in MPT cells from WT and α1-/- mice; # p < 0.05, anti-α2 shRNA vs. control shRNA, for total alpha domain expression in MPT cells from WT and α1-/- mice.