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Figure 8 | BMC Nephrology

Figure 8

From: Susceptibility to ATP depletion of primary proximal tubular cell cultures derived from mice lacking either the α1 or the α2 isoform of the catalytic domain of AMPK

Figure 8

Effect of knockdown of the α2 isoform on expression of the α1 and α2 isoforms and of total alpha domain in MPT cells from α1 -/- and WT mice. MPT cells from α1-/- mice, or their WT control, were infected with control (scrambled) shRNA or anti-α2 shRNA designed to knock down the expression of the α2 isoform of AMPK. The relative expression of the α1 and α2 isoforms of AMPK, as well as of the total α domain (α1 + α2), was evaluated in immunoblots of lysates from confluent MPT cells derived from the kidneys of α1-/- and α2-/- mice, and their WT controls. Upper panel: Representative immunoblot. Molecular weight markers (in kDa) are shown om the right of each immunoblot. Lower panel: Densitometric quantitation of three immunoblots using β-actin as a loading control. * p < 0.05, anti-α2 shRNA vs. control shRNA, for α2 isoform expression in MPT cells from WT and α1-/- mice; # p < 0.05, anti-α2 shRNA vs. control shRNA, for total alpha domain expression in MPT cells from WT and α1-/- mice.

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