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Figure 3 | BMC Nephrology

Figure 3

From: Gremlin induces cell proliferation and extra cellular matrix accumulation in mouse mesangial cells exposed to high glucose via the ERK1/2 pathway

Figure 3

Effects of Gremlin plasmid on cell proliferation and TGF-β1, CTGF and collagen IV expression in MMCs. MMCs were transfected with Gremlin (pEGFP-N1-Grem1) or corresponding negative control (pEGFP-N1) plasmids and incubated with HG (30 mM D-glucose) for 24 h. (A) Cell proliferation was examined using BrdU ELISA. Total (B) TGF-β1 and (C) CTGF proteins levels were determined using western blot analysis. (D) Culture medium was collected for measurement of collagen IV concentration by radioimmunoassay. NG: MMCs incubated with 5.5 mM D-glucose; NG + M: MMCs incubated with 5.5 mM D-glucose plus 24.5 mM mannitol; NG + V: MMCs transfected with pEGFP-N1 plasmid and incubated with 5.5 mM D-glucose; NG + P: MMCs transfected with pEGFP-N1-Grem1 plasmid and incubated with 5.5 mM D-glucose; HG: MMCs incubated with 30 mM D-glucose; HG + V: MMCs transfected with pEGFP-N1 plasmid and incubated with 30 mM D-glucose; HG + P: MMCs transfected with pEGFP-N1-Grem1 plasmid and incubated with 30 mM D-glucose. Values are expressed as means ± S.E.M. *P < 0.05,**P < 0.01, vs. NG, # P < 0.05, ## P < 0.01, vs. HG.

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