Figure 2

NKCC2 antibody characterization. A) Western blotting using a novel human NKCC2 specific antibody. The affinity-purified antibody (#594), detected a glycosylated smear characteristic of NKCC2 in human whole kidney (WK) and medulla samples, but not in rat inner medulla (IM). This signal was not observed following pre-incubation of the antibody with the immunizing peptide (P). B) Immunohistochemistry of a human kidney section using the novel NKCC2 antibody. Labeling was confined to the TAL. C) At higher magnification, the majority of NKCC2 signal was detected at the apical pole of TAL cells.