Characterization of 2,8-DHA crystals. (A) renal biopsy. Haematoxylin and myosin staining of a frozen section reveals interstitial inflammation surrounding multiple intra-tubular round brownish crystal that are birefringent under polarized light. (B, C) Urinary miscroscopy. Urinary sediment showing spherical brownish crystal that have a birefringent aqnd pseudo-Maltese cross appearance under polarized light. Magnifications: ×100 in A, ×40 in B and C.