Figure 3
From: Adenine phosphoribosyltransferase (APRT) deficiency: identification of a novel nonsense mutation
Characterization of APRT protein in erythrocyte lysate. Erythrocyte isolation from peripheral blood of a control and of patient by Ficoll/Paque density separation protocol. Purity was confirmed in flow cytometry, staining target cells with mouse anti-human anti-CD45 PE (leukocyte marker) (A) and anti-CD235a PerCP-eFluor710 (erythrocyte marker) monoclonal antibodies (B). (C) Western blot analysis performed by C-terminal and N-terminal APRT antibodies in a wild type control and in our patient.