Figure 4
Benazepril affects the TGF-β 1 , ILK and α-SMA expression in glomerular mesangial cell (GMC). Benazepril affects the expression of ILK (A) and α-SMA (B) mRNA in GMCs under HG conditions at different times, as determined by real-time RT-PCR. Benazepril affects the expression of ILK (C and D) and α-SMA (E and F) proteins in GMCs under HG conditions at different time points, as determined by western blot analysis. Densitometric quantification of the corresponding bands is performed using Kodak 1D Image software. The values are the means ± SE and are expressed relative to the control. n = 6. *, P < 0.05 vs. control; #, P < 0.05 vs. DN.