Figure 6

Benazepril affects Ang II signal pathways in cultured glomerular mesangial cell (GMC). (A) Ang II-induced ERK Thr²⁰²/Tyr²⁰⁴ and Akt Ser⁴⁷³ phosphorylation in the NG, MG, HG and ACEI were determined by western blotting. Representation of the quantitative analysis of the ratio of the phosphorylated/total ERK (B) or Akt (C) from three independent experiments. Densitometric quantification of the corresponding bands is performed using Kodak 1D Image software. The values are the means ± SE and are expressed relative to the control. n = 3. *, P < 0.05 vs. control; #, P < 0.05 vs. DN.