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Figure 7 | BMC Nephrology

Figure 7

From: The glomerular parietal epithelial cell’s responses are influenced by SM22 alpha levels

Figure 7

Double positive cells for PAX2 and α-SMA at × 630 original magnification. A) Double positive cells for PAX2 (red, nuclear) and α-SMA (blue/gray, cytoplasmic) at × 630 original magnification. A1-A3: representative images of PAX2/α-SMA double staining at × 630 original magnification in SM22α +/+ mice. A4-A6: fluorescent microscopic view of the A1-A3 bright-field view, where only PAX2 staining is seen because only the warp-red substrate is visible by fluorescent microscopy. A7-A9: representative images of PAX2/α-SMA double staining at × 630 original magnification in SM22α -/- mice. A10-A12: fluorescent microscopic view of the A7-9 bright-field view, where only PAX2 staining is seen because only the warp-red substrate is visible by fluorescent microscopy. A1, A4, A7, A10: day 0. A2, A5, A8, A11: day 7. A3, A6, A9, A12: day 14. B) Number of double positive cells for PAX2 and α-SMA along BBM/Bowman’s capsule (BC) length (mm) is increased in SM22α -/- mice, compared with SM22α +/+ mice.

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