SBF-SEM contrast imaging of glomerular glycocalyx. A: Section sequence (every 7.5 μm) from raw data stack (71 μm × 71 μm × 84 μm) The LaDy GAGa stained components can be seen as dark regions adjacent to the lower contrast of a conventionally processed TEM resin block (Additional file 2: Movie S3A). B: An inverted contrast image sequence (every 40o) of a 3D surface projection of the same Image Stack as above (Additional file 2: Movie S3B). C: The 3D surface projection has been intensity thresholded to reveal the LaDy GAGa (glycocalyx) stained vessels and structures (Additional file 4: Movie S3C). Scale Bars = 20 μm in length.