Serial block face scanning electron microscopy of a human glomerulus. A: Micrographs of complete field of view, slices 15 μm apart (z) at the edge of a glomerulus (Additional file 5: Movie S4A). B Micrographs of a podocyte (P) from inset in A to illustrate the sub-podocyte space (SPS). C = Capillary lumen, PUS = periphery urinary space, IPS = inter-podocyte space, and Exit is an exit region for an SPS (Additional file 6: Movie S4B). C: The same podocyte as in B and adjacent capillaries after highlighting and reconstructing (Additional file 7: Movie S4C). D: as C: The podocyte is differently oriented, and capillaries have been removed to display the SPS (Additional file 8: Movie S4D). Purple/Pink = Capillaries. Green = Podocyte cell body. Yellow = SPS (under the main cell body). Scale bar is 10 μm in all cases.