Figure 1

Immunohistochemistry and electron microscopy of healthy and CNF kidneys: A. Cortex of healthy kidneys with normal primary cilia (arrows in square) of proximal tubules (pt). B. CNF cysts of proximal tubules with columnar epithelium (CC) containing short and curly cilia (arrows). Immunostaining to α-tubulin, DAB and haematoxylin, scale bar 25 μm. C. Normal tubular primary cilia (arrow). D. “Broken” tubular primary cilia (arrow) in CNF cyst. Basal body (bb). Scale bar 0.1 μm. E. Ultrastructure of healthy tubular cell: oval nucleus (n), mitochondria (m), microvilli (mv) and basal body (bb) (square). Scale bar 1.2 μm. F. Ultrastructure of apoptotic cell in CNF cyst: dilated lumen (l) with colloid-like content, apoptotic cell with picnotic nucleus (n), reduced microvilli (mv)(square) and absence of cilia. Scale bar 1 μm.