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Figure 3 | BMC Nephrology

Figure 3

From: Shorter daily dwelling time in peritoneal dialysis attenuates the epithelial-to-mesenchymal transition of mesothelial cells

Figure 3

An in vitro protocol to investigate the dwelling time on the effect of mesothelial cells. Primary mesothelial cells (MCs) were incubated with conventional dialysate (4.25% glucose; Dianeal; Baxter) and diluted by one-half with culture medium for 8, 16, or 24 hours per day for 3 or 6 days (denoted as “8 hours”, “16 hours”, and “24 hours” groups). Primary MCs incubated with phosphate-buffered saline (PBS) solution or transforming growth factor-β1(TGF-β1) (3 ng/mL) served as the negative and positive controls, respectively. (dotted line: MCs were incubated with PBS and equivalent dilution with culture medium; thin solid line: MCs were incubated with 4.25% glucose dialysate and equivalent dilution with culture medium; thick solid line: MCs were incubated with TGF-β1 added in culture medium).

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