Figure 5
The effects of daily different dwelling times on cellular protein expression and cell survival. Expression of E-cadherin in mesothelial cells (MCs) was assessed on day 3, and cell counts were determined on days 3 and 6. A) The expression of E-cadherin, exhibited significant down-regulation with increasing dwell time (N = 4). B) The cell count decreased with a longer daily dwell time, where the maximal decrease was evident in the 24 hour group. On day 3, the 24 hour group demonstrated a significantly lower cell count compared to the phosphate-buffered saline (PBS) group. On day 6, the 16 and 24 hour groups demonstrated a significantly lower cell count compared to the PBS group. Additionally, the 24 hour group also demonstrated a significantly lower cell count compared to the transforming growth factor-β1 (TGF-β1) group (N = 4) (*P < 0.05; **P < 0.01; ***P < 0.005).