Figure 11

Chronic scarring following acute kidney injury. Central longitudinal kidney sections were prepared at 21 days after folic acid injury and stained with Mason’s Trichrome, which stains collagen blue. The upper panels show representative kidneys of mice sustaining minor injury and the lower panels are from mice sustaining severe injury; WT mice on the left, Clic4 null mice on the right of each pair. A. WT mouse with day 2 BUN of 30 mg/dl, day 21 BUN 20 mg/dl, and section scored at 2.8% fibrosis. B. Clic4 null mouse with day 2 BUN of 34 mg/dl, day 21 BUN 26 mg/dl, and section scored at 2.1% fibrosis. C. WT mouse with day 2 BUN of 350 mg/dl, day 21 BUN 54 mg/dl, and section scored at 38% fibrosis. D. Clic4 null mouse with day 2 BUN of 395 mg/dl, day 21 BUN 44 mg/dl, and section scored at 32% fibrosis.