Quantification of western blot signals for SMADs and PCNA. Digitized chemiluminescent signals as in Figure 13 were normalized to the GAPDH signal from the same lane and plotted as arbitrary units. Values from wild type (W) and Clic4 null (K) mice are shown at 0, 24, and 48 hours after folic acid injury as indicated. N = 8 for each group except for both 48 hour groups for which n = 7 (one mouse from each group was lost prior to analysis). A. pSMADs 2 and 3 normalized to GAPDH. B. Total SMADs 2 and 3 normalized to GAPDH. C. pSMADs 2 and 3 normalized to total SMADS 2 and 3. In panels A-C, the SMAD2 signal is shown with the solid bars, the SMAD3 signal with the hatched bars. D. PCNA. Error bars indicate SEM. Asterisk indicates comparison with P < 0.05. ns indicates not significant (P > 0.05).