Figure 3

Higher magnification images of CLIC4 in WT kidney. Pseudocolor as in Figure 2. Upper set of images (A-C) shows glomerular capillary loops, lower sets (D-F and G-I) show a portion of a proximal tubule and underlying peritubular capillaries. Panels A, D, and G (left column): CLIC4 signal alone; Panels B, E, and H (center column): merged image of the three markers (proximal tubule brush border in blue, endothelial cells in green, and nuclei in cyan); Panels C, F, and I (right column): merged image of all signals. Structures are indicated in the center image: L indicates proximal tubule lumen, N indicates proximal tubule cell nuclei, P indicates peritubular capillary. Note the close colocalization of CLIC4 (red) with IB4 (green) in both glomerular capillary loops (upper image set) and peritubular capillary (lower image sets). Also, note presence of CLIC4 (red) colocalizing with the brush border (blue) and in the nuclei (cyan) of the proximal tubule cells and in the nuclei of the peritubular capillary endothelial cells in the two lower image sets. Scale bar to the right of the upper panel represents 10 microns.