Figure 9From: Absence of chloride intracellular channel 4 (CLIC4) predisposes to acute kidney injury but has minimal impact on recovery Immunolocalization of CLIC4 in WT mice 48 hours after folic acid induced acute kidney injury. Vibratome sections stained with affinity-purified antibody to CLIC4 (red) plus markers for proximal tubule brush border (Lectin LTA, blue) and nuclei (DAPI, cyan). A set of lower magnification image is shown in the upper set (A-C) and a higher magnification set from the same kidney in the lower set (D-F). A and D (left column): CLIC4 signal (red) alone; B and E (center column): merged image of brush border (green) and nuclear (cyan) markers; C and F (right column): merged image of the CLIC4 signal with markers. In the upper set, solid arrow: CLIC4 is tightly polarized to the apical membrane of the dilated proximal tubule which has lost its brush border; solid arrowhead: nuclear localization of CLIC4 is less prominent than in uninjured kidney; open arrow: CLIC4 in a glomerular capillary loop; open arrowhead: CLIC4 in a peritubular capillary. In the lower set, L designates the lumen of the proximal tubule and N labels proximal tubule cell nuclei. A pertiubular capillary is present to the right.Back to article page