Immunolocalization of CLIC4 in WT mice 48 hours after folic acid induced acute kidney injury. Vibratome sections stained with affinity-purified antibody to CLIC4 (red) plus markers for proximal tubule brush border (Lectin LTA, blue) and nuclei (DAPI, cyan). A set of lower magnification image is shown in the upper set (A-C) and a higher magnification set from the same kidney in the lower set (D-F). A and D (left column): CLIC4 signal (red) alone; B and E (center column): merged image of brush border (green) and nuclear (cyan) markers; C and F (right column): merged image of the CLIC4 signal with markers. In the upper set, solid arrow: CLIC4 is tightly polarized to the apical membrane of the dilated proximal tubule which has lost its brush border; solid arrowhead: nuclear localization of CLIC4 is less prominent than in uninjured kidney; open arrow: CLIC4 in a glomerular capillary loop; open arrowhead: CLIC4 in a peritubular capillary. In the lower set, L designates the lumen of the proximal tubule and N labels proximal tubule cell nuclei. A pertiubular capillary is present to the right.