Figure 3

The contralateral kidneys of db RAS but not WT RAS mice develop progressive inflammation. A. Representative immunostaining of renal macrophages in paraffin section of contralateral kidneys. Macrophages were stained with anti F4/80 antibody. All images are taken at 200× magnification. B. Quantitative analysis of F4/80+ macrophages showed that despite lower baseline F4/80, there was increased infiltration in the contralateral kidney of db RAS mice but not WT RAS mice. C-D. RT-PCR analysis of CCL2 (C) and IL-6 (D) mRNA normalized to 18 s showed that contralateral kidney of db RAS mice produces more inflammatory cytokines and chemokines compared to WT RAS and db sham. Data are presented as means ± SE. † p < 0.05, †† p < 0.01 vs. db sham. * p < 0.05, ** p < 0.01, *** p < 0.001 vs. WT sham. §§§ p < 0.001 vs. WT RAS. Statistical significance was determined by 2-way ANOVA followed by Tukey adjusted post-hoc comparison to determine corrected p-values.