Figure 5

Angiotensin-II induced hypertension (Ang-II), unilateral nephrectomy (UNX), and combination of both angiotensin-II and unilateral nephrectomy (UNX + Ang-II) unable to re-produce all aspects of injury seen in the contralateral kidney of db RAS mice. A. Systolic BP were elevated to a similar extent in db RAS, db Ang-II and db UNX + Ang-II (p = ns) and not elevated in the db UNX mice. ** p < 0.01 vs. db sham. B. Plasma renin activity (PRA) was elevated only in the db RAS. PRA was assessed by angiotensin-I production. C. Representative histological images of contralateral kidney of db RAS and db sham mice, left kidney of db Ang-II mice, and remnant kidney of db-UNX and db UNX+Ang-II mice as stained with periodic acid schiff (PAS), fibronectin, and Masson’s Trichrome. PAS images are taken at 400× while fibronectin and trichrome images are taken at 200×. D. Mesangial matrix expansion was seen on db RAS, and to a lesser extent on the db UNX and db UNX + Ang-II, but not on db sham or db Ang-II. E. Interstitial fibrosis was increased to a similar extent in all models as assessed by trichrome stained section. F. Interstitial fibronectin deposition was increased to a greater extent in db RAS mice compared to all other models. For B, D, and F, * p <0.05 vs. db sham, db Ang-II, db UNX or db UNX + Ang-II. G. Urine albumin excretion is highest in db RAS and db UNX + Ang-II mice at 2 weeks post-surgery. * p < 0.05, ** p < 0.01 vs. db sham. § p <0.05 vs. db Ang-II. Albuminuria was measured in spot urine sample and normalized to urine creatinine. Data are presented as means ± SE. Statistical significance was determined by one-way ANOVA followed by Tukey adjusted post-hoc comparison.