Figure 6

Db RAS mice experienced higher level of inflammation compared to db mice with Angiotensin-II induced hypertension (Ang-II), unilateral nephrectomy (UNX), or both angiotensin-II and unilateral nephrectomy (UNX + Ang-II). A. Representative immunostaining of renal macrophages in paraffin section of contralateral or remnant kidneys. Macrophages were stained with anti F4/80 antibody. All images are taken at 200× magnification. B. Contralateral kidney of db RAS mice experienced higher increase of renal macrophage infiltration compared to db Ang-II, db UNX, or db UNX + Ang-II. ** p < 0.01 vs. db sham or db Ang-II, § p < 0.05 vs. db UNX or db UNX + Ang-II. Macrophages were stained with F4/80 antibody and quantified with NIS image analysis system. C-D. RT-PCR analysis of Ccl2 (C) and Il-6 (D) mRNA as normalized to 18 s showed that contralateral kidney of db RAS mice produces more inflammatory cytokines and chemokines compared to db Ang-II, db UNX, or db UNX + Ang-II. * p < 0.05 for db RAS vs. all other groups. E-F. Serum CCL2 (E) and IL-6 (F) concentration is elevated to a similar extent in db RAS and db UNX + Ang-II (p = ns). * p < 0.05 vs. db sham, db UNX, or db Ang-II. Data are presented as means ± SE. Statistical significance was determined by one-way ANOVA followed by Tukey adjusted post-hoc comparison.