Figure 2From: Properdin has an ascendancy over factor H regulation in complement-mediated renal tubular damage mRNA expressions of complement components belonging to the alternative pathway (AP) by proximal tubular epithelial cells (PTECs). PTECs were incubated with different concentrations of normal human serum (NHS; 5% or 25%) for 3 h. There were no significant changes in the mRNA expression of AP components (P, fH, fB, C3, C5 and C9) after the addition of up to 25% NHS. (a) Serum-free medium, (b) 5% NHS and (c) 25% NHS as evaluated by real-time polymerase chain reaction. Data are expressed as relative values compared with those for control cells after the addition of a serum-free medium for 0 h. The results are expressed as means ± standard errors of values from four independent experiments.Back to article page