Skip to main content

Table 1 Effect of sex steroids on Stx-1 cytotoxicity in human glomerular endothelial cells (HGEN). Cells were exposed to high and low concentrations of steroids for 1, 2 or 7 days followed by addition of varying concentration of Stx-1 for 72 hr. Cell survival was determined at the end of the experiment. % survival refers to percent of cells surviving after exposure to Stx-1 ± steroids. N = 9 each data point. Results are expressed as mean ± SEM.

From: Sex steroids do not affect shigatoxin cytotoxicity on human renal tubular or glomerular cells

HGEN Dose [Stx-1] gm/L Stx alone (% survival) Day 1 (% survival) Day 2 (% survival) Day 7 (% survival)
Estradiol 1 μM -5 54 ± 4 49 ± 3 49 ± 6 48 ± 6
  1 μM -8 90 ± 7 86 ± 6 85 ± 8 95 ± 6
  1 μM -11 102 ± 8 102 ± 10 105 ± 4 91 ± 8
  100 pM -5 54 ± 4 51 ± 3 55 ± 3 58 ± 4
  100 pM -8 90 ± 7 87 ± 6 96 ± 9 85 ± 9
  100 pM -11 102 ± 8 96 ± 6 103 ± 11 115 ± 11
Progesterone 1 μM -5 54 ± 4 51 ± 4 50 ± 4 45 ± 6
  1 μM -8 90 ± 7 84 ± 9 85 ± 8 95 ± 7
  1 μM -11 102 ± 8 100 ± 4 103 ± 6 95 ± 7
  30 nM -5 54 ± 4 53 ± 3 53 ± 4 52 ± 5
  30 nM -8 90 ± 7 91 ± 9 92 ± 7 94 ± 9
  30 nM -11 102 ± 8 102 ± 7 104 ± 7 100 ± 7
Testosterone 1 μM -5 54 ± 4 58 ± 5 55 ± 2 56 ± 3
  1 μM -8 90 ± 7 91 ± 8 92 ± 9 94 ± 10
  1 μM -11 102 ± 8 103 ± 12 95 ± 8 102 ± 7
  30 nM -5 54 ± 4 52 ± 4 54 ± 4 55 ± 3
  30 nM -8 90 ± 7 86 ± 6 90 ± 6 94 ± 8
  30 nM -11 102 ± 8 96 ± 6 100 ± 5 104 ± 8