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Table 2 Effect of sex steroids on Stx-1 cytotoxicity in human glomerular epithelial cells (HGEC). Cells were exposed to high and low concentrations of steroids for 1, 2 or 7 days followed by addition of varying concentration of Stx-1 for 72 hr. Cell survival was determined at the end of the experiment. % survival refers to percent of cells surviving after exposure to Stx-1 ± steroids. N = 9 each data point. Results are expressed as mean ± SEM.

From: Sex steroids do not affect shigatoxin cytotoxicity on human renal tubular or glomerular cells

HGEC Dose [Stx-1] gm/L Stx alone (% survival) Day 1 (% survival) Day 2 (% survival) Day 7 (% survival)
Estradiol 1 μM -5 54 ± 4 51 ± 4 51 ± 3 48 ± 5
  1 μM -8 90 ± 7 79 ± 10 94 ± 8 86 ± 5
  1 μM -11 102 ± 8 96 ± 10 107 ± 5 95 ± 8
  100 pM -5 54 ± 4 53 ± 4 52 ± 3 50 ± 4
  100 pM -8 90 ± 7 88 ± 8 89 ± 6 83 ± 7
  100 pM -11 102 ± 8 106 ± 7 96 ± 9 98 ± 9
Progesterone 1 μM -5 54 ± 4 54 ± 49 56 ± 5 54 ± 2
  1 μM -8 90 ± 7 83 ± 9 90 ± 9 90 ± 5
  1 μM -11 102 ± 8 101 ± 3 101 ± 6 103 ± 7
  30 nM -5 54 ± 4 50 ± 6 58 ± 4 50 ± 5
  30 nM -8 90 ± 7 86 ± 4 92 ± 6 78 ± 9
  30 nM -11 102 ± 8 97 ± 7 105 ± 7 93 ± 7
Testosterone 1 μM -5 54 ± 4 53 ± 4 55 ± 4 50 ± 4
  1 μM -8 90 ± 7 90 ± 11 90 ± 7 84 ± 6
  1 μM -11 102 ± 8 103 ± 9 100 ± 7 105 ± 5
  30 nM -5 54 ± 4 59 ± 6 59 ± 4 54 ± 9
  30 nM -8 90 ± 7 84 ± 8 89 ± 5 82 ± 7
  30 nM -11 102 ± 8 98 ± 6 106 ± 8 97 ± 6