Table 2 Effect of sex steroids on Stx-1 cytotoxicity in human glomerular epithelial cells (HGEC). Cells were exposed to high and low concentrations of steroids for 1, 2 or 7 days followed by addition of varying concentration of Stx-1 for 72 hr. Cell survival was determined at the end of the experiment. % survival refers to percent of cells surviving after exposure to Stx-1 ± steroids. N = 9 each data point. Results are expressed as mean ± SEM.
From: Sex steroids do not affect shigatoxin cytotoxicity on human renal tubular or glomerular cells
HGEC | Dose | [Stx-1] gm/L | Stx alone (% survival) | Day 1 (% survival) | Day 2 (% survival) | Day 7 (% survival) |
|---|---|---|---|---|---|---|
Estradiol | 1 μM | -5 | 54 ± 4 | 51 ± 4 | 51 ± 3 | 48 ± 5 |
1 μM | -8 | 90 ± 7 | 79 ± 10 | 94 ± 8 | 86 ± 5 | |
1 μM | -11 | 102 ± 8 | 96 ± 10 | 107 ± 5 | 95 ± 8 | |
100 pM | -5 | 54 ± 4 | 53 ± 4 | 52 ± 3 | 50 ± 4 | |
100 pM | -8 | 90 ± 7 | 88 ± 8 | 89 ± 6 | 83 ± 7 | |
100 pM | -11 | 102 ± 8 | 106 ± 7 | 96 ± 9 | 98 ± 9 | |
Progesterone | 1 μM | -5 | 54 ± 4 | 54 ± 49 | 56 ± 5 | 54 ± 2 |
1 μM | -8 | 90 ± 7 | 83 ± 9 | 90 ± 9 | 90 ± 5 | |
1 μM | -11 | 102 ± 8 | 101 ± 3 | 101 ± 6 | 103 ± 7 | |
30 nM | -5 | 54 ± 4 | 50 ± 6 | 58 ± 4 | 50 ± 5 | |
30 nM | -8 | 90 ± 7 | 86 ± 4 | 92 ± 6 | 78 ± 9 | |
30 nM | -11 | 102 ± 8 | 97 ± 7 | 105 ± 7 | 93 ± 7 | |
Testosterone | 1 μM | -5 | 54 ± 4 | 53 ± 4 | 55 ± 4 | 50 ± 4 |
1 μM | -8 | 90 ± 7 | 90 ± 11 | 90 ± 7 | 84 ± 6 | |
1 μM | -11 | 102 ± 8 | 103 ± 9 | 100 ± 7 | 105 ± 5 | |
30 nM | -5 | 54 ± 4 | 59 ± 6 | 59 ± 4 | 54 ± 9 | |
30 nM | -8 | 90 ± 7 | 84 ± 8 | 89 ± 5 | 82 ± 7 | |
30 nM | -11 | 102 ± 8 | 98 ± 6 | 106 ± 8 | 97 ± 6 |