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Table 3 Effect of sex steroids on Stx-1 cytotoxicity in human proximal tubule cells (HPT). Cells were exposed to high and low concentrations of steroids for 1, 2 or 7 days followed by addition of varying concentration of Stx-1 for 72 hr. Cell survival was determined at the end of the experiment. % survival refers to percent of cells surviving after exposure to Stx-1 ± steroids. N = 9 each data point. Results are expressed as mean ± SEM.

From: Sex steroids do not affect shigatoxin cytotoxicity on human renal tubular or glomerular cells

HPT Dose [Stx-1] gm/L Stx alone (% survival) Day 1 (% survival) Day 2 (% survival) Day 7 (% survival)
Estradiol 1 μM -5 54 ± 4 49 ± 4 50 ± 4 46 ± 5
  1 μM -8 90 ± 7 81 ± 7 83 ± 7 84 ± 9
  1 μM -11 102 ± 8 97 ± 5 99 ± 7 107 ± 7
  100 pM -5 54 ± 4 49 ± 4 60 ± 4 49 ± 6
  100 pM -8 90 ± 7 95 ± 8 90 ± 7 87 ± 8
  100 pM -11 102 ± 8 97 ± 6 103 ± 5 92 ± 9
Progesterone 1 μM -5 54 ± 4 56 ± 4 52 ± 3 60 ± 5
  1 μM -8 90 ± 7 99 ± 9 81 ± 7 91 ± 9
  1 μM -11 102 ± 8 107 ± 8 101 ± 5 106 ± 8
  30 nM -5 54 ± 4 50 ± 6 52 ± 3 54 ± 4
  30 nM -8 90 ± 7 86 ± 4 83 ± 5 90 ± 5
  30 nM -11 102 ± 8 97 ± 7 105 ± 5 92 ± 7
Testosterone 1 μM -5 54 ± 4 57 ± 3 49 ± 4 58 ± 5
  1 μM -8 90 ± 7 92 ± 7 89 ± 5 91 ± 8
  1 μM -11 102 ± 8 94 ± 5 97 ± 6 100 ± 6
  30 nM -5 54 ± 4 51 ± 4 64 ± 7 57 ± 3
  30 nM -8 90 ± 7 98 ± 8 97 ± 7 85 ± 5
  30 nM -11 102 ± 8 109 ± 10 102 ± 6 103 ± 7