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Table 3 Effect of sex steroids on Stx-1 cytotoxicity in human proximal tubule cells (HPT). Cells were exposed to high and low concentrations of steroids for 1, 2 or 7 days followed by addition of varying concentration of Stx-1 for 72 hr. Cell survival was determined at the end of the experiment. % survival refers to percent of cells surviving after exposure to Stx-1 ± steroids. N = 9 each data point. Results are expressed as mean ± SEM.

From: Sex steroids do not affect shigatoxin cytotoxicity on human renal tubular or glomerular cells

HPT

Dose

[Stx-1] gm/L

Stx alone (% survival)

Day 1 (% survival)

Day 2 (% survival)

Day 7 (% survival)

Estradiol

1 μM

-5

54 ± 4

49 ± 4

50 ± 4

46 ± 5

 

1 μM

-8

90 ± 7

81 ± 7

83 ± 7

84 ± 9

 

1 μM

-11

102 ± 8

97 ± 5

99 ± 7

107 ± 7

 

100 pM

-5

54 ± 4

49 ± 4

60 ± 4

49 ± 6

 

100 pM

-8

90 ± 7

95 ± 8

90 ± 7

87 ± 8

 

100 pM

-11

102 ± 8

97 ± 6

103 ± 5

92 ± 9

Progesterone

1 μM

-5

54 ± 4

56 ± 4

52 ± 3

60 ± 5

 

1 μM

-8

90 ± 7

99 ± 9

81 ± 7

91 ± 9

 

1 μM

-11

102 ± 8

107 ± 8

101 ± 5

106 ± 8

 

30 nM

-5

54 ± 4

50 ± 6

52 ± 3

54 ± 4

 

30 nM

-8

90 ± 7

86 ± 4

83 ± 5

90 ± 5

 

30 nM

-11

102 ± 8

97 ± 7

105 ± 5

92 ± 7

Testosterone

1 μM

-5

54 ± 4

57 ± 3

49 ± 4

58 ± 5

 

1 μM

-8

90 ± 7

92 ± 7

89 ± 5

91 ± 8

 

1 μM

-11

102 ± 8

94 ± 5

97 ± 6

100 ± 6

 

30 nM

-5

54 ± 4

51 ± 4

64 ± 7

57 ± 3

 

30 nM

-8

90 ± 7

98 ± 8

97 ± 7

85 ± 5

 

30 nM

-11

102 ± 8

109 ± 10

102 ± 6

103 ± 7