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Figure 2 | BMC Nephrology

Figure 2

From: Lipoprotein lipase in hemodialysis patients: indications that low molecular weight heparin depletes functional stores, despite low plasma levels of the enzyme

Figure 2

Evaluation of the consistency of data from four separate studies by repeated assay of frozen samples. Samples had been obtained and treated as described in the methods section and then stored frozen at -70°C in three earlier studies of plasma LPL during infusion of conventional heparin or dalteparin in control subjects or in dialysis patients [12,22,23] and the present study. Samples from the 0, 15 and 30 min time points were thawed and assayed for LPL activity and mass. Panel A shows the LPL activities at 15 and 30 min recorded on the second assay, as a function of the value recorded on the original assay. Regression analysis gave a slope indicating that the repeated value was 113 % of the original (r = 0.94, p < 0.0001). Panel B shows the increase of LPL activity over the baseline samples plotted against the increase of LPL mass for the 30 min samples. For this, values from the second assay were used (LPL mass was not determined in some of the earlier studies). A regression analysis, excluding samples for which the increase in LPL mass was less than 100 μg/mL, returned a slope of 0.46 ± 0.05 mU/ng LPL (r = 0.94, p < 0.001). Same symbols as in Fig 1.

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