Figure 3

Effect of RANTES/CCL5 and Met-RANTES on proliferation of human MCIncubation of human MC for 24 hours with various concentrations of RANTES/CCL5 (10 ng/ml, 100 ng/ml, 250 ng/ml, 1 μg/ml, 10 μg/ml) and Met-RANTES (10 ng/ml, 100 ng/ml, 250 ng/ml, 1 μg/ml, 10 μg/ml) has no effect on proliferation of MC under standard conditions. To induce expression of chemokine receptor CCR1 MC were prestimulated with IFN-γ (20 ng/ml), TNF-α (25 ng/ml) and IL-1β (10 ng/ml) for 24 hours prior to adding test substances. Cell proliferation was analyzed with the MTT assay as described in Methods. Cells growing under standard conditions served as control. Changes in proliferative activity are given as relative values to the respective controls. Each bar represents a mean ± SEM of 7 parallel incubations for each condition. Comparable results were obtained in three series of independent experiments.