Figure 8

Effect of RANTES/CCL5 and Met-RANTES on Fas-induced cell death of human MC in cell cycle analysis by flow cytometry. Percentage of apoptotic cells was analyzed in cell cycle analysis by flow cytometry after staining with propidium iodide. Histograms represent cell counts (y-axis) versus DNA content (x-axis) with the percentage of apoptotic cells containing sub-G1 DNA indicated. (A) Cell cycle analysis of MC was performed after serum starvation and stimulation with IFN-γ for 48 hours. (B) Apoptosis was induced subsequently by Fas ligation. Prior to serum starvation the expression of chemokine receptor CCR1 was induced by pretreatment with a combination of IFN-γ (20 ng/ml), TNF-α (25 ng/ml) and IL-1β (10 ng/ml) for 24 hours. (C,D) RANTES/CCL5 250 ng/ml and Met-RANTES 250 ng/ml have no effect on Fas-induced cell death of MC.