Figure 1

Histopathology of protein overload kidney from control and experimental animals. Parts A, C, E, G are all the same magnification (scale bar 0.1 mm) for comparison of relative sizes among groups. Similarly, parts B, D, F, H are all the same magnification (scale bar 0.1 mm) and each figure has the justiglomerular apparatus (JGA) denoted by an arrow head to show the vascular pole. A) Survey view showing normal cortex (200×) for NOD.B10 treated with saline (PAS stain). B) Higher magnification of saline-treated NOD.B10 showing normal glomerular structure (400×). JGA is shown (arrow) and tubular pole is opposite. C) BAS-treated NOD.B10 (PAS stain). Survey view showing increased staining in all 4 glomeruli accentuating Glomerular lobules (200×). D). Higher magnification of a BSA-treated NOD.B10 glomerulus showing expansion extending from the JGA region (arrows) to the peripheral mesangium accentuating the glomerular lobularity (400×). E) Saline treated NON (PAS stain) survey view of 5 glomeruli with preserved parenchyma (200×). Glomerular hyaline is just visible. F). Higher magnification of saline-treated NON shows intracapillary hyaline thrombi (arrows, 400×). G) BAS-treated NON (PAS stain) survey view (200×) of cortex with 4 glomeruli showing changes similar to those seen in BSA-treated NOD.B10 mice. No residual intracapillary thrombi are present. H) Higher power view of two BSA-treated NON glomeruli with varying degrees of mesangial expansion. JGA is indicated by arrow (400×).