The table reports the modification of vascular permeability; following in vivo treatment with EDTA and TNFα (see Methods section). The rat right kidney was in vivo perfused with trypan blue solution; washed with saline; removed; homogenized and centrifuged. The supernatants were run on a spectrophotometer at 540 nm wavelength. The data was then expressed as μg dye retained per weight (g) of fresh kidney. Maximum dye retention (dye perfusion without washing) yielded a value of 619 ± 24.7.