Figure 7
Roscovitine increases p21 with no effect on apoptosis. MDCK cells were seeded 24 h before treatment. Roscovitine (at the concentrations indicated) was added to the cells for 24 h and then the cells were harvested and subjected immunoblotted with p21, β-actin, and PARP antibodies. PARP control (+PARP) represents cells treated with the DNA damaging agent doxorubicin showing the lower molecular weight cleavage band. QM is serum-free media; ser is serum-containing media.