At-RA treatment reduces proinflammatory mediators and VCAM-1 expression in renal tissue. A) Proinflammatory cytokines mRNA analysis at day 13. Quantitative real-time RT-PCR of total RNA isolated from kidneys from all experimental groups. Results normalized to β-actin are expressed as mean ± SD; *P < 0.001 vs. HgCl2-injected rats. B) Expression of VCAM-1 in total renal lysates estimated by Western Blot. On the right, quantification of VCAM-1 expression is expressed as the mean ± SD of the densitometry values, relative to actin;*P < 0.001 vs. HgCl2-injected rats.